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Application rate trials with a nuclear polyhedrosis virus to control Spodoptera frugiperda (Smith) on maize Anais da SEB
Cruz,Ivan; Figueiredo,Maria L. C.; Valicente,Fernando H.; Oliveira,António C..
Different concentrations of nuclear polyhedrosis virus were applied as aqueous suspensions using a tractor mounted or a backpack-manual sprayer to control Spodoptera frugiperda (Smith) larvae on maize (Zea mays L.). Larval mortality varied with the application equipment. For the tractor-mounted sprayer application of at least 2.5 x 10(12) P.I.B./ha was necessary to attain a control comparable to that obtained with the backpack-manual sprayer application (70.2%). For this application method it can be used the dose of 2.5 x 10(11) PI.B./ ha, butNPV persistence was very short. A greaterNPV persistence was obtained with doses over 1.25 x 10(12) P.I.B./ha (93.4% mortality). The occurrence of parasitoids in the experimental area increased larval mortality rate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Fall armyworm; Baculovirus; Biological control.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591997000100019
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Bacillus thuringiensis survey in Brazil: geographical distribution and insecticidal activity against Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) Neotropical Entomology
Valicente,Fernando H.; Barreto,Marliton R..
A total of 3408 strains of Bacillus thuringiensis were collected from 1448 soil samples in 10 Brazilian states, four different geographical regions, covering 96 counties. These strains were evaluated against Spodoptera frugiperda (J.E. Smith) larvae. Only 62% killed between 81% and 100% and 1758 caused no mortality. Highest proportion of efficient strains (larval mortality above 75%) was found from the total isolated per region in the South Region (16.6%), followed by Western Central (3.1%), Southeast (1.1%) and Northeast Region (0.4%).
Tipo: Info:eu-repo/semantics/report Palavras-chave: Insect; Bacteria; Native isolate; Biological control; Pathology.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2003000400014
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Does Bacillus thuringiensis have adverse effects on the host egg location by parasitoid wasps? Rev. Bras. entomol.
Nascimento,Priscilla T.; Fadini,Marcos A.M.; Valicente,Fernando H.; Ribeiro,Paulo E.A..
ABSTRACT This study investigated the interaction between two pest biological control agents, the parasitoid wasp Trichogramma pretiosum (Hymenoptera: Trichogrammatidae) and the entomopathogen Bacillus thuringiensis (Bacillales: Bacillacea) (Bt). The aim of this study was to evaluate if the presence of Bt (formulated products Agree®, Dipel® and HD1 and HD11 strains) interferes in the oviposition preference of T. pretiosum to eggs of Helicoverpa zea (Lepidoptera: Noctuidae). Using an olfactometry test, the eggs of H. zea were bathed with the commercial formulations, with the Bt suspensions or distilled water, and offered to the parasitoid wasps in order to evaluate parasitism. The results showed that H. zea eggs sprayed with commercial formulations and Bt...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Helicoverpa zea; Trichogramma pretiosum; Oviposition preference; Zea mays.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262018000400260
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Effect of Baculovirus spodoptera isolates in Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD Neotropical Entomology
Barreto,Marliton R.; Guimaraes,Cláudia T.; Teixeira,Flávia F.; Paiva,Edilson; Valicente,Fernando H..
The total of 22 Baculovirus isolates surveyed in different corn producing regions in Brazil were used against fall armyworm, Spodoptera frugiperda (J.E. Smith). The viruses were purified and their suspensions were used to feed fall armyworm larvae from 4th and 5th instar. The mortality rate was checked daily and the infected larvae were frost after death, what generally occurred between the 5th and 7th day after virus ingestion. The 22 Baculovirus isolates were used in six concentrations (from 10³ to 10(8) polyhedra/ml) and one check treatment with water. Mortality rate, larval period, pupal period, pupa weight and lethal concentration (LC50) were determined for all isolates. Significant differences were found among all isolates and different...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insect pathology; Fall armyworm larvae; PCR.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100010
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Identificação através de PCR dos genes CryI de cepas de Bacillus thuringiensis Berliner eficientes contra a lagarta do cartucho, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) Anais da SEB
Valicente,Fernando H.; Barreto,Marliton R.; Vasconcelos,Maria J. V. de; Figueiredo,José E.F. de; Paiva,Edilson.
Fall armyworm, Spodoptera frugiperda (J. E. Smith), is the most important maize insect pest in Brazil and its damage can reduce yield up to 34%. The objective of this work was to utilize the PCR technique to Identify Bacillus thuringiensis (B.t) strains that control S. frugiperda in maize crops. Bioassays showed that 16 strains were very effective against S. frugiperda, 15 of them collected from soil samples and one (T09) obtained at the Institute Pasteur. The DNA of these strains were probed with cryI general primers and their proteins were analised by SDS-PAGE eletrophoresis. All strains present positive results for cryI genes. To further characterize these strains, specific cryI primers were employed. PCR technique showed that some strains harbour the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; CryI genes; Biocontrol; Fall armyworm.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80592000000100018
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Insecticidal activity of culture supernatants from Bacillus thuringiensis Berliner strains against Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) larvae Anais da SEB
Barreto,Marliton R.; Loguercio,Leandro L.; Valicente,Fernando H.; Paiva,Edilson.
Novel vegetative insecticidal proteins (Vips) identified in the supernatant of Bacillus thuringiensis (B.t.) cultures have shown to provide adequate control over a wide spectrum of economically important crop pests. To evaluate the potential applicability of these proteins against fall armyworm (Spodoptera frugiperda Smith) larvae, the most important insect pest for tropical maize, the characteristics and mortality effects of culture supernatants from five B.t. strains were investigated. Striking differences among strains were detected, not only in terms of efficiency in killing the insect, but also regarding to mortality effects of heated and non-heated supernatants, which were used to distinguish the heat-sensitive protein-derived insecticidal fraction...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Biological control; Fall armyworm; Vegetative insecticidal proteins; Vip genes.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591999000400010
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Levantamento dos inimigos naturais da lagarta do cartucho do milho, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), na região de Cascavel, PR Anais da SEB
Valicente,Fernando H.; Barreto,Marliton R..
A survey of the natural enemies of S. frugiperda larvae was done during the years of 1992/93 and 94/95, in Cascavel region, Parana State. The most frequent parasitoids were Campoletis sp. (Hymenoptera: Ichneumonidae), which reached 47.0% of parasitism and Archytas marmoratus (Diptera: Tachinidae), which reached 15.4%. Of all Baculovirus found, 10.8% were NPV (nuclear polihedrosis virus) and only 1% was GV (granulosis virus).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Parasitoids; Baculovirus; Fungi.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591999000200018
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Use of by-products rich in carbon and nitrogen as a nutrient source to produce Bacillus thuringiensis (Berliner)-based biopesticide Neotropical Entomology
Valicente,Fernando H.; Mourão,André H.C..
The amount and sources of carbon and nitrogen used to produce Bacillus thuringiensis (Berliner)-based biopesticide may influence the quality of the final product. The objective of this research was to test different levels of carbon and nitrogen: medium 1 - 1.5% maize glucose + 0.5% soy flour, medium 2 - 3.0% maize glucose + 1.0% soy flour, medium 3 - 1.0% maize glucose + 3.0% soy flour and medium 4 - Luria Bertani (LB) + salts (FeSO4, ZnSO4, MnSO4, MgSO4). The seed culture was produced in LB medium plus salt, under agitation (200 rpm) for 18h at 30ºC. The strain 344 of Bt was used (B. thuringiensis var tolworthi - belonging to the Embrapa's Bt Bank). The pH was measured at regular intervals. and After culturing for 96h, the pH of the four tested media was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spodoptera frugiperda; Biocontrol; Insect pest management; Microbial insecticide.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2008000600012
Registros recuperados: 8
Primeira ... 1 ... Última
 

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